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Due to sample quantities, the exact same set of control DNA could not be used twice.
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Quantities of human 18S rRNA gene copies by qPCR were relatively constant showing consistent loading of human cells on the swabs with good stability of DNA and fluctuations in DNA levels were not due to sample quality (Figure 1).
Prior further evaluation, the data was additionally normalized to definite starting concentration in order to remove the errors introduces due to uneven sample quantities.
Six specimens were excluded from comparison due to insufficient sample quantity to perform sequencing (BAL 2, bladder urine 1), insufficient sample to perform the genotyping analysis (kidney urines 2 and 5) or genotypes that could not confirm the specimen to be from the same patient as the blood (bladder tumor 4 and kidney tumor 7); these are designated n/a in Table 1.
Indeed, due to sampling constraints, the quality and quantity of RNA available for library preparation may be very heterogeneous and limited [ 12, 13].
Numbers rounded to the nearest 100 due to sampling methodology.
However, a major drawback of 2DE is sensitivity primarily due to the sample quantity requirements of protein visualization methods and losses associated with peptide/protein extraction from the gel.
Although one physiological theory [ 5] suggests that anoxia (or hypoxia) might be the cause for NDEs, Parnia and colleagues [ 3] found a higher mean pO2 in peripheral blood; however, due to an insufficient sample quantity, a univariate analysis was not performed.
PCA analysis was not conducted on acrylic samples due to the small quantity of samples and IR spectra available.
In our cohort, a 35-kDa antigen is likely to be CRALBP although its identity wasn't confirmed (due to insufficient quantity of samples) if confirmed, these AAbs would be found to be 2 times more frequent in individuals with AMD than in controls.
The regions encoding BEX2 with 3 CpG sites and cXorf23 with a single CpG site were each run in a single sample well due to DNA quantity limitations (N = 59 including the original 46 profiled on BeadChip).
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