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We have shown, however, that there is a large difference in residence time of the PET imaging signal, likely due to internalization and subsequent residualization of the 89Zr label versus rapid efflux of the 124I label.
When stimulated by ligand this repression is lifted due to internalization and degradation of PTCH1.
Further, after engulfment cells lacked the chance to develop an apoptotic phenotype due to internalization and metabolic degradation.
We also have in vitro data suggesting that VEGFR3 is slightly reduced under flow mainly due to internalization and degradation.
Continuous treatment of oocytes with BFA resulted in the nearly complete loss of Kir3.2-HA after 8 h due to internalization and recycling.
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MiRNAs that are present in serum or plasma are collectively called circulating miRNAs, which are extremely stable in boiling water, prolonged room temperature incubation, or repetitive freezing-thawing cycles and highly resistant to plasma RNase activity due to internalization in vesicles and binding to circulating proteins [ 12, 13].
To see if the loss of surface GlyR antibody IgG was due to internalization, surface bound GlyR antibody was visualized (green) and scored at different times and compared with cells fixed and permeabilized in 0.3% Triton™ X-100 so that internal IgG could be visualized with Alexa Fluor® goat anti-human IgG (red).
However, due to differences in their relaxativity and a reduction in water interaction due to internalization within the cell any differences in T1 cannot be predicted.
Mechanistically, increased expression of adhesion molecules in response to MPs may be due to internalization of MPs by endothelial cells, thereby inducing expression of cell adhesion molecules and activation of the nuclear factor-κB [ 42].
It is therefore possible that at high levels of CCL20, the chemokine receptor CCR6 is desensitized due to internalization and/or phosphorylation [25].
Therefore, the upper limit to the decrease in net BRET one could expect due to internalization is within this range.
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