For gonorrhea, both PCR and culture were used; however, only culture was used at MSHC during the study period and while both PCR and culture were used at VIDRL, the analysis was stratified by test type, to account for any difference in trends that may have been observed due to different test sensitivities.
However, growth inhibition doses (EC50) have enormous variations due to different test parameters with values ranging from 0.007 to 53 mg/l (Gellert et al. 1999).
More detailed information of Rubia triterpenes is listed in the following Table and Figure, Mp refers to Melting point; means to the Specific Rotation of the compounds (due to different test conditions, the data may be various).
This may be due to different test characteristics of IGRA vs LPA, or the fact that commercial IGRA assays employ a mycobacterial antigen (CFP-10) that we did not use in the present LPA assay.
Part of this discrepancy could be due to different groups testing for stemness using different assays.
In particular the design of the two TBMs is discussed pointing out the common original architecture and the differences in the design choices due to different functionality, test necessities and adaptation to the ITER boundary conditions.
In addition, due to different variant tests reported in each paper (a total of 16 combinations for four amino-acid substitutions) and different expression and assay systems used between different laboratories, the results were not consistent and were difficult to compare (Hanna et al, 2000; Li et al, 2000; McLellan et al, 2000; Shimada et al, 2001; Aklillu et al, 2002).
These differences are potentially due to different accessions being tested between studies.
In addition, liquefaction of the sputum specimen over time could contribute to false-positive readings, perhaps due to different absorption by the testing device.
