If amplification continued to prove unsuccessful, likely due to developmental stage specific gene expression, PCR experiments were repeated with gDNA and gDNA-specific primers.
To control for changes in gene expression due to developmental delay, worms were "staged" based on expression patterns of developmentally regulated genes identified in unexposed worms using linear regression models.
It is however believed that it may be due to developmental changes around the site.
It might be due to developmental regulation of proline synthesis in plants (Lehmann et al. 2010).
To avoid regional discrepancy due to developmental stages, only midsections of flag leaves were utilized.
However, due to developmental factors, behavior data collection and interpretation can be severely compromised if inappropriate designs are applied.
These divergent findings may be due to developmental changes in the relation between ELS and HPA-axis functioning.
Open image in new window Fig. 5 a d A patient with scoliosis due to developmental anomalies in the thoracolumbar junction.
The extra spleens found in other animals, including rabbits, Chinese hamsters, chickens, and capuchin monkeys, meanwhile, may be due to developmental problems.
In general, children are referred to centers of treatment and diagnosis due to developmental delays, learning disabilities, or social interaction problems perceived by parents, teachers, or other professionals who follow the children into their routine.
Gross and fine motor skills, communication, cognition, and independence in activities of daily living vary by age, and it may be difficult to distinguish between treatment effect and change due to developmental maturation.
