To rule out the possibility that the observed phenotypes are due to construct-dependent interruption of endogenous genes involved in heart function, we analyzed two independent transgenic mouse lines using different approaches.
The use of the same implants does not allow variation due to construct design.
To enable us to distinguish effects due to construct length from effects due to the introduction of interchain disulfide bonds, we created a corresponding set of mutants based on the minimal IKKβ binding region NEMO-44 111, NEMO-44 111NEMO44–111(designatedMO44–111(des2C/C54), NEMO44 111 des3C/L107C), aNEMO44 111 des2C/C54C54/L107C).
In other words, differences in performance cannot be due to different constructs being measured from year to year.
The oppression of women due to gender constructs, not allowing women basic citizenship rights such as to vote (for example) was often robustly justified in peer review religious and scientific journals, as well as affirmed by both religious canon and secular legal courts, including the Supreme Court.
This was not due to the constructs failing to express as can be seen from the inset, the levels of DG-GFP, despite being a transient expression, are relatively similar.
Comparison in function was limited due to divergent constructs.
Staining of TβRII between wildtype and transfected cells differs only in intensity and not structurally, indicating that the observed spatial organization is not due to expression constructs alone.
The latter three constructs contain the CDE1 motif plus either the CGC (F10 and F08) or CCG motif (F23); the level of gene expression due to these constructs was reduced by the addition of cysteine.
Besides interference with pain, responsiveness in function cannot be compared due to divergent constructs – the MPI asks about very specific functions in its activity dimension whereas the SF-36 covers physical tasks more generally, e.g. ambulation.
