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Higher pulse numbers led to inconsistent results, probably due to beginning tissue carbonization, and thereby reduced antigen uptake 36.
However, we cannot exclude that the decreased CALUX activity observed at 10−4 M for all compounds, except nNP alone, might be a response due to beginning cytotoxicity.
This view is supported by the change in particle morphology following uptake observed in some samples by BF-TEM (reduction in size and rounding of the needle- and plate-like shapes of the as-synthesized particles), which we surmise is due to beginning particle degradation.
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