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This may have partially been due to background problems.
The low number of detectable transcripts on the LGTC platform is mainly due to background problems, causing negative control spots to occasionally give high signals.
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Microarrays have several limitations as those due to background or cross-hybridization problems.
Here, BTBR generates visual artifacts due to background reconstruction oversmoothness.
Figure 3 Visibility thresholds due to background luminance [[17]].
Such artifacts are generated due to background reconstruction oversmoothness.
The slow phase is probably due to background drift.
However, by adding the two effects, one can explain as much as 50% of the difference between two platforms and this can be even more for home-spotted arrays were the numbers of detectable transcripts are often reduced due to local background problems.
On more severe cases, the CAD system had higher bias due to problems in the background noise filtering methods.
Due to problems with high background observed using alternative western blotting protocols, non-reducing anti-PrxIV blots of SP cell derived material were performed exactly as described previously (Tavender et al, 2008).
The recognition of SINs in actual factory scenes is a challenging problem due to complicated background and low-quality of characters.
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Justyna Jupowicz-Kozak
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