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Extracellular stimulation may therefore have activated several axons, accounting for the large IPSCs elicited by stimulation in some cultures and their longer time course than those of sIPSCs and the AP-IPSCs recorded during dual recordings, when the output of only one neurone was activated and recorded.
In electrophysiological experiments, dual recordings were generally made from the same larva.
IPSC amplitude varied substantially between trials in all dual recordings.
Paired T-tests were used for kinetic parameters in dual recordings, LTP, and CP93129 experiments; unpaired T-tests were used for comparison between ages in dual recordings and between layers in minimal stimulation experiments.
These AP-IPSCs, whether from dual recordings or extracellular stimulation, were generally of similar duration to sIPSCs in the same HEK293-GABAAR cells.
Wilcoxon paired signed-rank test (www.stattools.net) was used to test between amplitudes in dual recordings due to non-normal distribution of amplitudes.
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Hippocampal slices from Tg and WT mice were prepared simultaneously and placed in a chamber outfitted with dual-recording equipment, thereby minimizing day-to-day variability in slice preparations and recordings.
Dual extracellular recordings, local application of tetrodotoxin and recordings in M1 micro-sections indicate that the activity originates within deep layers V/VI.
Either by connecting an antenna, attaching the cable without a box (fine if you don't get premium channels) or by renting a CableCard — or two, for dual simultaneous recordings.
To study cellular mechanisms of the active state onsets, which underlay the slow waves, we made simultaneous LFP and quadruple, triple, or dual intracellular recordings from nearby neurons (<200 μm lateral distance, Fig. 5).
Hidaka et al. [ 39] examining rat retina using dual patch recordings and tracer labels, and demonstrated dendrodendritic gap junctions and electrotonic coupling of alpha ganglion cells of the same type, including On-center cells.
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