The dual real-time RPA demonstrated a 100% diagnostic agreement with the real-time PCR on 4 PRV strains and 37 clinical samples.
The dual real-time RPA assay provides an alternative useful tool for rapid, simple, and reliable detection and differentiation of PRV, especially in remote and rural areas.
The dual real-time RPA assay performed in the single tube was capable of specific detecting and differentiating of the wild-type PRV and gE-deleted vaccine strains, without cross-reactions with other non-targeted pig viruses.
The objective of this study was to develop a dual real-time recombinase polymerase amplification (RPA) assay using exo probes for the detection and differentiation of pseudorabies virus (PRV).
To provide new insights into the dispersion of PARV4 in healthy persons, we assessed the frequency of PARV4 viremia in a cohort of blood donors by using a dual real-time assay.
But the coordinates of all 3-vector points including the control points to be interpolated are now taken to be dual, not real, numbers.
The speech intelligibility levels were measured using 01 dB Dual Channel Real Time Frequency Analyser Type Symphonie, and analysed using acoustics software to give the RASTI values.
Animals were exposed to a 112-dB sound pressure level of broad-band white noise (1 20 kHz) measured by a dual channel real time frequency analyzer (type 2144, Brüel and Kjær, Nærum, Denmark) for 3 h.
We selected 10 SNPs for conversion into Taqman® MGB dual-probe real-time PCR SNP assays (Table 3).
DNA for sub-clade specific TaqMan® MGB dual-probe real-time PCR SNP assays was extracted by a simplified heat lysis protocol described in Keim et al. (2000).
To divide the A.Br.008/009 TEA group into 2 lineages (A.Br.008/011 and A.Br.011/009; Figure), we converted the canSNP A.Br.011 assay (17 ) into a TaqMan MGB dual-probe real-time PCR (Applied Biosystems, Foster City, CA, USA).
