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Colorimetric quantification was performed with a microplate reader (550; Bio-Rad, Hercules, CA, USA) using dual readings at 450/570 nm.
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Dual-reading CR was shown to provide better detection of (simulated) chest lesions than single-reading CR [ 4].
Dual-reading CR systems are based on transparent detector material and employ light collection optics in the front and the back side of the detector (Fig. 2).
The model can give the variation of dual-laterolog readings with time.
Cells were lysed 48 h post-transfection, and dual-luciferase readings were performed using the Genecopoeia Luc-Pair miR Luciferase Assay Kit on a TD-20/20 TD-20/20ter (Turner Designs, SunnyvaLuminometer).
The dynamic resistivity responses are sensitive to the variations of formation water saturation, hence an effective method to evaluate reservoirs is suggested by history matching the dynamic dual-induction logging readings.
Readings from dual excitation at 340/380 nm with an emission wavelength of 510 nm were recorded using a Perkin-Elmer Fluorescence Spectrometer LS-55.
For BIA, subjects lay supine while plastic electrodes were placed on their right hand and foot and a trained technician took dual resistance and reactance readings with a Quantom Impedance Meter (RJL Systems, Michigan).
The purity and concentration of RNA were determined from OD260/280 readings using a dual beam UV spectrophotometer (Eppendorf AG, Hamburg, Germany).
Firefly/ Renilla luciferase activity ratios were calculated by the software, using the readings obtained with the dual luciferase assay system (Promega).
Incident photon to current conversion efficiency (IPCE) is determined using PVE300 Bentham Instruments Ltd , Reading, Berkshire, UK), with dual xenon/quartz halogen light source, measured in DC mode and no bias light is used.
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