Sentence examples for dual labelling from inspiring English sources
For dual labelling of replication tracts, exponential cell cultures were pulse-labelled with 25 μℳ CIdU (Sigma-Aldrich) followed by 250 μℳ IdU (Sigma-Aldrich), for 20 min each.
"The customer's opinion is what matters most and now they have spoken and confirmed our belief that the dual labelling system is the simplest and clearest.
So we are urging major food retailers and manufacturers to end the debate, listen to the customers and commit to the dual labelling scheme".
Even without moving to a single European price list, currency conversion and a period of dual labelling pose difficulties as the psychologically important threshold price-points such as 1.99 are unlikely to convert neatly into euros.Supply and demandThe second level on which the euro will force firms to reassess their strategy is in their relationship with suppliers.
We found that PfHRP2 is mainly located in the erythrocyte cytosol of infected erythrocytes, however, dual labelling studies suggest that the location of a sub-population of the PfHRP2 molecules overlaps with that of the food vacuole-associated protein, P-glycoprotein homologue (Pgh-1).
For dual labelling, slides were rinsed and non-specific protein blocked with milk powder and Triton-X 100 in PBS.
Two sets of images are shown; higher magnification dual-labelling with calcofluor (blue) and antibody labelling contrast-enhanced by changing the hue angle to a reddish-pink colour, and antibody immunofluorescence labelling in green.
Our findings show the feasibility of dual-labelling tissues and casts of both litter-feeding (Lumbricus terrestris) and soil-feeding (Aporrectodea caliginosa) earthworms using the same method.
In all dual-labelling analyses, we confirmed that there was no bleed-through between the two wavelengths.
Dual-labelling immunofluorescence showed NMDAR1 co-localised with CD206-positive cells, whereas PGP9.5 and glutamate were predominately expressed by CD206-negative cells (resident tendon cells) (Fig. 4).
However, with scores of 2.2 to 3.9 AU, compared to staining in saline and IFA sections, which yielded scores of 1 or less, dual-labelling confirmed CHOP expression in astrocytes and microglia.
