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In kidney, lung, and spleen, the dual label experiment using C-labeled non-hydrolyzable retinyl ether along with H-retinyl palmitate [18] showed that these tissues took up equal amounts of C- and H-label indicating that REH activity of LPL is not essential for retinoid uptake in these tissues.
This means in a binary classification that a single label prediction is correct if the label is the correct one, a dual label is always correct, and an empty prediction is always incorrect.
These low level isolated MCT positives did not result in false-positive detection of targets in a dual label system.
Dual label immunofluorescence was also employed to quantify the intensity of pCREB in cortical neurons according to the previously described procedure [26].
Dual label hybridizations on the microarrays were performed with a reference sample that was identical for all the hybridizations in this study.
In the dual label system presented here, detection of a target (marker) must be the result of making two positive calls, one for each of the MCTs correlated with that target locus.
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Copy number analysis of hybridised probes is based on a dual-label approach by comparing the intensity of Cy3-labelled MAPH probes amplified from test samples co-hybridised with similarly amplified Cy5-labelled reference MAPH probes.
A dual-label experimental design was employed for the microarray hybridisations with Cy3-labelled test samples competitively hybridised to a common Cy5-labelled pooled-reference per array.
To assess whether RNA payloads remained annealed to cell-internalizing aptamers during internalization into NALM6 cells, dual-label colocalization studies were performed with Cy5 on the targeting module and AF488 or 3WJdB on the payload module.
The functionality of the platform is demonstrated with a dual-label homogeneous polymerase chain reaction (PCR) assay for the detection of Salmonella using a Magda CA Salmonella assay kit.
Application of LaBr3(Ce) scintillator coupled to photosensor with high photon detection efficiency and excellent energy resolution will allow dual-label imaging to monitor simultaneously the cardiac perfusion and the molecular targets under investigation during the heart therapy.
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