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To evaluate the analytical performance of the Bio-Rad Variant II HbA1c dual kit assay.
Of these, 12 showed the R132H mutation, 25 showed dual KIT and PDGFRA amplification and 10 showed PDGFRA amplification only.
The number of α-globin genes is also provided N.A. not available, N.M. not measurable Each sample was analyzed by cation-exchange high-performance liquid chromatography (HPLC) using the Hb Variant I or II systems with either the 6-min β-Thalassemia short or the Dual Kit program (BioRad Laboratories, Hercules, CA, USA).
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Cells were harvested and reporter activity measured using a Dual Luciferase kit (Promega) and a Luminescent β-galactosidase detection kit II (Clontech), on an LmaxII 384 luminometer (Molecular Devices).
There is also a dual light kit, with two lights, a Sweep and two reflecting cards, for $268.
All reagents are components of the Dual Luciferase Kit (Promega Cat. #E1960).
LucR and LucF activities were measured using the Dual Luciferase kit from Promega.
48 h after transfection, cells were collected and luciferase activities assayed using the Dual Luciferase Kit from Promega.
24 or 48 h after transfection, the cells were harvested to conduct a firefly and Renilla luciferase activity assay using a dual luciferase kit (Promega).
48 h after transfection, cells were washed with pre-chilled PBS and lysed in passive lysis buffer (Dual Luciferase kit, Promega).
24 h after transfection, cells were washed twice with ice-cold PBS and lysed according to the Dual Luciferase Kit protocol (Promega, WI).
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