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Although we have not used a dual expression vector vaccine expressing VP2 and VP5, it is clear from our studies that VP2 alone was indeed able to provide full protection in the IFNAR −/− model.
So far, only Quyen et al. [ 18] had chose a dual expression plasmid system to express an active lipase, but the dual expression plasmid was derived from pET22b and its construction process was complex.
Such dual expression systems could be used to co-express immune enhancer elements [ 52] or immunogenic baculovirus elements together with HIV-1 Gag VLPs to improve VLP immunogenicity.
To assess their ROMT activities in vivo, we synthesized VrROMT and SbROMT3 following codon-optimization and expressed the VrROMTsyn and SbROMT3syn genes using a dual expression vector system.
Miyata, S. T., Unterweger, D., Rudko, S. P. & Pukatzki, S. Dual expression profile of type VI secretion system immunity genes protects pandemic Vibrio cholerae.
Dual expression peaks at P6 and P50 (cluster 2) were observed for genes required for morphogenesis, assembly and organization of cilia and other cell projections, and cell morphogenesis.
The dual expression of gRNAs/SaCas9 in these cell lines resulted in more efficient HBV genome cleavage.
We have developed a dual expression system for the simultaneous overexpression of two proteins in Bacillus subtilis.
We have constructed a dual expression vector for the production of recombinant proteins in both Escherichia coli and insect cells.
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The dual-expression system is comprised of codon-optimized Cullin3 and Rbx1 genes co-expressed from a single pET-Duet-1 plasmid.
pOmni has two technical advantages compared with the only pro/eukaryotic dual-expression vector pDual GC (Agilent, USA) that we know.
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