As the coverage increases, the reactions are limited to dissociative adsorption through single and dual attachments.
We show that hTIM-1 can act as a dual-attachment receptor to recruit EBOV to cell surface by interacting directly with GP and the PS on the viral envelop.
Open image in new window Figure 5 hTIM-1 serves as a dual-attachment receptor for EBOV and identification of hTIM-1 Ig V residues that impact EBOV GP-dependent entry.
Immunostaining for each assay was assessed by two personnel using a dual viewing attachment to an Olympus BH-2 light microscope Southend-onn Sea, Essex, UK).
At least five fields were viewed simultaneously using a light microscope at x20 magnification and a representative field was then assessed by both researchers at x40 magnification using a dual viewing attachment.
Emission light was collected from the bottom port of the microscope and passed through the Dual-View attachment to simultaneously acquire GFP and mCherry emission.
Cytoplasmic HRGβ1, membrane-phosphorylated erbB2 and nuclear-phosphorylated AKT immunostaining were assessed by two personnel using a dual-viewing attachment to an Olympus BH-2 light microscope (Jencons Ltd ,Leighton Buzzard, Bedfordshire, UK).
We present a structure-derived model for dual GTPase membrane attachment that highlights the potential ability of Rab GTPases to reach binding partners at a significant distance from the membrane via their unstructured and membrane-anchored, hypervariable domains.
Godwin and co-workers from PolyTherics subsequently attached MMAE to these bis-sulfone reagents to prepare trastuzumab MMAE conjugates with DARs close to 3. Importantly, these conjugates did not suffer from the instability inherent to the equivalent maleimide conjugates (see below), likely due to the dual sites of attachment.
So the dual-head shaver attachment that promises an "ultra close shave" does not, in fact, shave ultra close (see above photo) — just to get that out of the way.
Thus, Cdc20 has a dual function: until kinetochore attachment, Cdc20 contributes to MCC formation and thus APC inhibition, while after attachment Cdc20 acts as the APC activator.
