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The final detection device measured approximately 10 mm × 40 mm × 3 mm and contained two detection channels for dual analyses.
The extent of potential contamination is not known, but we attempted to mitigate this error through running dual analyses with MTAT exposure dichotomized by both intention-to-treat and per-protocol methodology.
However, our separate analyses using both of these criteria only resulted in a difference of 1.5% of all children included as myopic, in keeping with other dual analyses [ 13, 41], and is low either way when compared with Asia or North America.
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The same nuisance regressors adopted in the dual regression analyses (see above), with the addition of global signal time course were used in the seed-based analyses.
For more precise localization, dual immunohistochemical analyses were performed to detect TUNEL activity and TLR3 expression.
Therefore, we performed dual FISH analyses with S0524M13 in combination with other BACs from the set that was used to define the Atlantic salmon chromosomes and integrate them with the linkage groups in the genetic map (Phillips et al. 2009).
When this sdY-containing plasmid was used as a probe in dual FISH analyses with BACs known to hybridize to Ssa02, Ssa03, or Ssa06, the results revealed that the sdY gene was indeed on the chromosomes to which SEX had been mapped in these male lineages.
Cytogenetic dual FISH analyses showed that a BAC containing the sdY gene hybridized not only to its expected location on European Atlantic salmon chromosomes (i.e., Ssa02) (Artieri et al. 2006) but also to Ssa03 and Ssa06, where SEX had been mapped in Tasmanian families (Eisbrenner et al. 2014).
The use of a plasmid probe that contains the sdY gene without flanking genomic sequences in dual FISH analyses showed that the sdY gene did indeed localize to the chromosomes where SEX had been mapped in different Tasmanian Atlantic salmon families.
Recovery values of TBDF through the cleanup procedure averaged 50% and, using this recovery value and TBDF as an external standard, dual ion analyses indicated that 40 ppm tetra- and 4 ppm pentabrominanted dibenzofuran were produced based on the PBB level used in the pyrolysis experiments.
In all dual-labelling analyses, we confirmed that there was no bleed-through between the two wavelengths.
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