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In experiment 1, a preliminary experiment, data were collected daily for 4 days after DT administration.
In experiment 2, data were collected during the first 24 hours after DT administration because in experiment 1, most luteolytic activity occurred during this time.
A DT administration regimen supramaximal for completely ablating epidermal Langerhans cells in Lang-DTR mice reduced PGP 9.5-immunoreactive total innervation and calcitonin gene related peptide-immunoreactive peptidergic nociceptor innervation.
Subsequent acute cell ablation with local or systemic DT administration circumvents any potential developmental compensation.
DT administration at 16 weeks post-transplant ablated 80.5% of hCNS-SCns.
In the present study, we tested this hypothesis and demonstrated that hCNS-SCns survived and migrated, and 80.5% of the transplanted cells were ablated after DT administration.
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All animals in the study were reassessed on the BBB/BMS, and the ladderbeam task 1 week after DT or saline administration.
DT: Look, Establishment.
In these B6.Latfl-dtr-mice, the administration of DT allowed the selective ablation of CD4+ T cells (Figure S2) [23].
More importantly, we found that the transfer of Treg cells one month later still reduced both the number of CD4+ T cells (Figure S2) and the CD8+ T cell recovery (Figure 3E) mimicking the results observed upon the late administration of DT in hosts with Lat-DTR CD4+ T cells.
To further investigate the emerging role of DCs in NKT cell activation, we took advantage of the CD11c-DOG system that allows depletion of DCs after administration of DT (Probst et al, 2005; Hochweller et al, 2008).
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