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22 samples failed for DST assay were excluded.
Regarding drug susceptibility testing (DST), of all 24 positive MTB culture samples, three samples did not have DST results because the strain failed to grow.
We conclude that Waldminghaus and Skarstad failed to identify DSTs not because of an improvement in the ChIP methodology, but because of lower data quality.
In the LPA-based algorithm, low MDR-TB risk patients did not have initial drug susceptibility tests (DST); this was done only when 1st-line TB treatment failed, resulting in substantial delays to MDR-TB treatment.
In Lima, DST is only performed on isolates from patients in whom treatment failed or suspicion of MDRTB is high.
WHO recommended a microbial culture and drug susceptibility test (DST) for all re-treatment TB cases and new PTB+ cases who failed to convert sputum examination results at the end of a second month of follow-up (30).
rpoB Sequencing was done for one isolate which failed to amplify for MAS-PCR 25/300 isolates (8.3%) had discrepant DST results between MODS and LJ for INH or RIF.
Currently, it is not clear why DST-primed whole splenocytes fail to induce tolerance whereas CD4+ T-cells enriched from DST-primed splenocytes do.
This suggests the importance of early request for culture and DST than awaiting the outcome of extended category II treatment among patients for whom category I failed.
Then failed".
All failed.
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