After overnight air drying, staining of CK+ cells was performed using the Epimet® kit (Micromet, Munich, Germany).
After fixation in 80% chilled acetone, drying, staining with 0.1% crystal violet and washing, the plates were examined under the microscope.
However, after development, during the time period utilized for drying, staining, and marking the spots of interest in the analytical TLC plate by pencil, the separated components were exposed to the ambient laboratory environment.
Thin sections (80 100 nm) were cut, post-stained with saturated uranyl acetate (20 min), rinsed with water, dried, stained with Reynolds' lead citrate (10 min) and dried again.
The slides were air dried, stained with 4,6- diamidino-2-phenylindole (DAPI; 2 M) for 10 min in the dark, rinsed again with distilled water, and finally air dried.
Brain sections were mounted, dried, stained with thionin, dehydrated and coverslipped.
After 12 days, the wafers were scraped, dried, stained with toluidine blue, and examined under a 40× objective.
Aspirates were macroscopically evaluated for caseation and smears were air dried, stained with Wright stain and examined cytologically by a pathologist.
Sections were mounted on coated slides, dried, stained (if appropriate) with cresyl violet (FD Neurotechnologies, Columbia, MD, USA) and coverslipped with Depex (Sigma-Aldrich, St . Louis MO, USA).
Slides were cleared, dried, stained with 5% Giemsa solution (Electron Microscopy Sciences, Hatfield, PA), mounted with Permount, and visualized using a compound microscope.
The slides were air dried, stained with 10 % Giemsa for 10 minutes, and examined by bright-field microscopy at 1000× magnification.
