Sentence examples for drying mounting from inspiring English sources

Embedded samples were cut using a microtome (10 µm sections; Microm Heidelberg HM330, Germany), cleared with xylene and mounted in fast drying mounting medium (United Biosciences, Australia).

Secondary antibodies were applied for 2 3 h (4 °C) and sections washed in PBS before drying, mounting and cover slipping using Vectashield (Vector Laboratories, Peterborough, UK).

After fixation, samples were dehydrated through a series of increasing concentrations of ethanol, followed by critical-point drying, mounting on aluminium stubs and coating with platinum.

Cells were then dehydrated for 10 min in 35%, 50%, 70 %, 80 %, 95 and 100% ethandl, followed by critical point drying, mounting onto stubs and sputter coated with palladium/gold, and viewing with a Hitachi S5000 scanning electron microscope at 20 kV.

Finally, sections were transferred onto a slide in a drop of PBS and, after drying, mounted with Vectashield mounting medium containing DAPI to counterstain cell nuclei (Vector Laboratories).

Specimens were then serially dehydrated in ethanol and subjected to freeze drying, mounted on metal stubs and sputter-coated with gold.

Samples were dehydrated, critical point dried, mounted on support stubs with fast-drying silver paint and lightly sputter coated with platinum.

Filters were stained with 10x SYBR Gold (Invitrogen, USA) for 15 min, dried, mounted to slides using VectaShield (Vector Laboratories, USA).

After staining, the slides were washed, dried, mounted with mounting medium (Neomount, Merck) and analyzed using a laser scanning confocal microscope (LSM 510 META, Zeiss).

Sections were transferred onto slides, dried, mounted with 0.1% paraphenylinediamine in 90% glycerol/PBS (PPD), and imaged with a confocal microscope (Nikon).

Skeletal fragments (2 3 cm diameter) were cleaned, dried, mounted on stubs, and imaged with a Hitachi S-800 Field Emission Scanning Electron Microscope operated at 15 KV with a minimum resolution of 20 mm.