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The result is a bottle of pink-tinted juice that's bubbly, effervescent — and almost grassy — at first spritz before drying down to a delicate, uncomplicated floral.
After labeling, the MeDIPs and inputs were combined and then divided into two equal volumes before drying down to 187.5 µL each.
Dipalmitoyl-PI5P (DiC16–PI5P) was purchased from Echelon Biosciences and after drying down in Eppendorf tubes was sonicated for 3 × 30 s in a Decon Ultrasonics sonicating bath.
After drying down under vacuum and redissolving in 2 vol of 60% ethanol, the extract was further cleaned by passage through a 60 mg Oasis hydrophilic-lipophilic balanced (HLB) cartridge.
The protein-depleted serum was concentrated using 4-ml 5 kDa molecular weight cutoff Vivaspin centrifugal concentrators (Sartorius, Goettingen, Germany) at 3200 g and 10 °C, the buffer was exchanged with distilled water and further concentrated before drying down in a SpeedVac (Thermo Scientific, Waltham, MA, USA).
The Zn concentrations of the standard and spike solutions were accurately matched for these measurements and potential matrix effects due to different acid concentrations were avoided by drying down and re-dissolving suitable aliquots of the concentrated Zn DS and London Zn stock solutions in 0.1 M HNO3 prior to analysis.
Similar(54)
The cleaned extract is aliquoted into two equal portions and the supernatant is dried down again.
Aliquots of the polar and non-polar phase were immediately dried down after extraction.
In fact, as the scent dried down, it did begin to smell familiar.
A 50 μl aliquot of supernatant was taken and dried down in a speed vacuum.
The supernatants were used for bacterial isolation and also dried down for DNA extraction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com