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The tissue was then blotted dry, weighted, and followed by homogenization in 1.5 mL cold buffer (50 mM potassium phosphate and 1 mM EDTA, pH 7) and centrifugation at 10,000 g for 15 minutes at 4°C.
One part of each tumour was dry weighted, counted in a gamma counter (Wallac, Turku, Finland) and the percentage of injected dose per gram tumour (ID g−1, %) was calculated.
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The dried seeds were weighted (DW, dry weight).
As in Binsl et al., the contributions of the individual samples were weighted by the dry weight wsample for each sample [ 8]. (6) MV O 2 heart = ∑ w sample * MV O 2 sample ∑ w sample For all six experimental groups, the comparison of MVO2 estimated with the LIPSSS method (from the model parameters Pdil and JTCA) with the "gold standard" oxygen measurements is shown in Figure 3.
All the seeds and seed traces from 25 berries of the mixture were extracted, counted (seed number, SN), weighted (total seed fresh weight, TSFW), dried at 80°C for 48 hours and weighted again (total seed dry weight, TSDW).
Filters were dried at 95°C for 24 h and weighted to calculate algal dry weight.
The dried sample was re-weighted to calculate the percent dry weight.
For determination of biomass dry weight, the yeast cells in the pre-weighted test tubes were dried in a heating oven for 72 h at 105 °C.
The ears were dried for 24 h in dry oven, weighted again and the weight defined as dry weight.
The extracted lipids were weighted and oil content per 100 g of cell dry weight was calculated.
For cellular dry weight, a known volume of cultivation broth was filtered using pre-weighted filters; these were washed with two volumes of distilled water and dried to constant weight at 105°C for 24 h.
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