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The phenol (1 equiv) was dissolved in dry MeCN (~6 mL/mmol) in a dry vial under argon atmosphere.
After completion of the coupling, the resin was transferred into a sealed dry vial containing 3 mL of dry CH2Cl2 then, 1-hexene (0.4 mmol) and Grubbs' second generation catalyst 2 (0.01 mmol) were added consecutively.
To the dry vial, 50 µL of MOX reagent (2% methoxyamine-HCl in pyridine; Thermo Fisher Scientific, Ashville, NC) was added, and the vial was capped with a teflon-lined screw cap and then heated for 2 h at 80°C in a dry block heater.
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For analysis of acetaminophen, blood was distributed into dry vials and left to coagulate for 20 min at room temperature before centrifugation and handling as described below.
After freeze drying vials were sealed at 4 °C until required.
An oven dried microwave vial under argon atmosphere was charged with the acid (1.3 equiv), dry DCM (2 mL/mmol), N,N-diisopropylethylamine (5.5 equiv) and BTFFH (1.5 equiv).
Adult anophelines from pyrethrum spray catches and larval surveys were identified morphologically to species [ 22] and preserved dry in vials containing a desiccant (silica gel).
(B ) Box plot of changes in ab4 spike frequency to vials containing flies stimulated with dry air, or vials containing flies that had been stimulated with apple cider vinegar (p = 0.03; t-test, n = 5 per condition).
The solvent was evaporated using a nitrogen stream until dry and the vial was kept under vacuum for 1 h to 2 h to remove residual solvent.
For group 2 (n = 133), the first swab was placed in transport medium and the second swab was placed in the empty vial (dry swab).
The enantioselective alkylation of aldehydes was performed in an overnight oven dried Pyrex glass vial equipped with a septum and a dried magnetic stir bar.
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