Samples from the caudae epididymal fluid were used for sperm morphology examination and aliquots were used to prepare dry smears and fixed in formol saline (4 5% aqueous solution of buffered formaldehyde).
In vitro calibration with a moving dried smear of erythrocytes illustrated a linear relation between the output of the frequency-voltage converter and erythrocyte velocity for a wide range of velocities and magnifications.
SAF (Sodium acetate-Acetic acid-Formalin) preserved specimens and air dried smear were taken to the EHNRI's (Ethiopian Health and Nutrition Research Institute) parasitology department for analysis.
How it froths, foams, cracks, drips, dries, smears and slides.
Dried smears may be stored and transported at ambient temperature.
Dried smears were analyzed by optical microscope in immersion objective and images were captured using the digital system (Nikon Trinocular Microscopic Unit, Model E-200, Japan).
Semen pellets were washed twice, and two slides (one was stored for security) were prepared and dried at room temperature for 60 min. Dried smears were submersed in Carnoy's solution (three parts methanol to one part acetic acid).
10 ml aliquots were then taken at 1 h, 6 h, 18 h, with an additional 1 ml being harvested to prepare air dried smears, which were fixed in methanol at -20°C.
The air dried smears were incubated in the substrate for 1 hour at 37°C, rinsed in distilled water, counterstained in solution V (mixture of 77.1 mL of 0.2 M sodium phosphate, 122.9 mL 0.1 M nitric acid, and 2 g methyl green) for 5 minutes, and rinsed in distilled water.
Cryptosporidium parvum and I. belli oocysts were recovered using the modified Ziehl Neelsen Stain (Casemore et al. 1985) method and when dry, the smear was examined for identification of oocysts under microscope using 40× and 100× objective.
