Exact(4)
For cars, dry filters, with pleated resin impregnated paper or, in a very few instances, felt elements are employed.
Dry filters were placed into scintillation vials with 2 ml scintillation fluid.
Radioactivity bound to dry filters was determined by liquid scintillation counting.
Incubate for 10 min at 37°C, precipitate charged tRNA by adding 5 ml of ice-cold 5% trichloroacetic acid (TCA), filter through GF/C filters (Millipore), dry filters, add 4 ml Ready Protein scintillation cocktail (Beckman Coulter, USA) and count. 1 U of an aminoacyl-tRNA synthetase enzyme is equal to the amount of the enzyme that charges one picomole of cognate tRNA per second.
Similar(56)
After water washing, the oil may be dried by heating in a vacuum or by filtering through a dry filter-aid material.
A reddened, sweaty area about an inch in diameter appears on the skin, and a collection pad of dry filter paper is taped over it to absorb the sweat for half an hour.
An alternative is to use a solid support, such as dry filter paper media.
A piece of dry filter paper was placed at the top of the specimen to separate the soil specimen from the dry porous stone.
Similar procedure was followed to obtain the 12.5 mg/ml and 6.25 mg/ml concentrations, using dry filter paper dispersion method.
To avoid the inoculative freezing of pupae, the surface of the pupae was dried at the rearing temperature, and moist filter paper in the Petri dish was replaced with dry filter paper before the exposure to −20°C.
Total counts per sample were determined by spotting 10 µl of RNA on a dry filter.
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