Sections (5 μm) were fixed on glass slides for 2 × 2 min in methanol:acetone (50 50), allowed to air dry and expression of p16 examined using a p16 monoclonal antibody (1 1000 for 1 h; Ab-7; Labvision, Fremont, CA, USA) and the catalysed signal amplification system (CSA System; DakoCytomation, Cambridgeshire, UK) according to manufacturer's instructions.
The largest portion of TACs in Dry exhibited rare expression (RPKM < 3).
When the lichen starts to dry, the gene expression profiles are similar to those observed during rehydration.
All RNA samples had a 28S/18S ratio ≥1.8 and were stored at −80°C before shipment on dry ice to Expression Analysis Inc. (Durham, NC).
All samples had a 28S/18S ratio ≥ 2.0 and were stored at −80°C before shipment on dry ice to Expression Analysis Inc. (Durham, NC, USA; www.expressionanalysis.com) or their use in real-time polymerase chain reaction.
Figure 2A shows that, compared to the low amount present in dry seeds, DAG2 expression in seeds was much increased following water uptake in the dark (2 and 4 fold, respectively, at 12 and 24 hrs).
Dietary exposures of 5 and 13.5 μg MeHg/g dry wt increased expression of genes cytochrome c oxidase subunit I (coxI) and cytoplasmic superoxide dismutase (sod) associated with mitochondrial metabolism and production of reactive oxygen species (ROS) in liver and skeletal muscle.
10.7554/eLife.08411.005 Figure 2. In the dry season analysis, expression patterns of the two genotypes are highly correlated for most clusters while for the few other clusters, differences between the genotypes are poorly explained by the models (high model MSEs for clusters with low correlation).
Similar to GCR2, GCL1 and GCL2 are expressed in dry seeds and the expression of all three genes decreases once seeds have germinated [9].
A kitchen scene, it depicts a few subtle disasters — a boy taking a cookie from a jar on a high shelf while falling off a stool; sibling coercion; a mother with a glazed expression drying a dish as a sink overflows into a puddle at her feet.
