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For measuring pH, 100 ml of the sample taken in a clean dry beaker stirred well until reached the stable value.
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The solutions of PVP and AgNO3 were prepared separately by dissolving appropriate amounts of AgNO3 (10 and 25 mM AgNO3 in 10 ml) and PVP in distilled water and in well-cleaned dry beakers at room temperature.
An aliquot of 1.1 mL of DMSO was added to the dried out beaker.
An accurately weighed amount of the gel (0.1 g) was transferred into a clean dry 100 mL beaker and about 80 mL of ethanol was added.
The pellets were washed in ddH2O, centrifuged again, then the pellets were resuspended in ddH2O, transferred to a weighed beaker, dried at 105°C until constant weight.
The catalyst was further dried by placing the beaker in an oven maintained at 120 °C for at least 48 h prior to use.
The supernatant was discarded and the gels transferred into a beaker and dried at 80 °C for 24 h to produce xerogels.
To determine biomass production, wet fungal biomass that was collected after 60 h of growth was placed in a pre-weighed beaker and dried at 105 °C to a constant weight.
The coarse part of the sample remaining in the beaker was dried completely at 110°C and was weighed to yield the weight, by difference, of the removed fine part (< several tens μm).
This suspension was transferred to a pre-weighed beaker and dried at 105°C for 24 h.
All in vitro fertilizations took place in dry 1-L plastic beakers, with batches containing an average of 66 eggs (± 0.596SE, n = 360) exposed to a 5- μL sperm-extender sample activated by 500 mL of Imsa river water (at natural temperatures of 3°C).
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