It combines the advantages of both dry SERS assays based on plane films deposited with silver colloids and wet SERS assays utilizing cuvettes or capillary tubes.
The inhibition experiments were performed by first diluting peptide stock solutions (assays with HFIP) or dissolving dry peptides (assays without HFIP) in the buffer, followed by the addition of insulin.
According to our results, all dry chemistry assays still gave too large biases for pool 1 (35 μmol/L); for pool 2 (70 μmol/L) the biases are within the minimal bias specification criterion.
Explants were weighed wet, frozen, lyophilised, reweighed dry then assayed for sulphated glycosaminoglycan (sGAG) and hydroxyproline content.
Detached leaf drying assay was reported to be an easy assay for testing large numbers of plants for their dehydration stress avoidance [ 60].
Following sap collection, the filter paper bullet was weighed, dried and assayed for Cd.
Pooled medium concentrated 12-fold by dialysis and freeze-drying was assayed for sulphated glycosaminoglycans, using shark cartilage chondroitin sulphate as standards.
Ten undamaged, mature leaves were harvested for measurement of leaf area and specific leaf area (leaf area/dry mass), and a further ten leaves were harvested, dried and assayed for condensed tannins and total phenolics as described in [ 34].
For assays on dry fibronectin, the granulocytes adhesion was carried out as above except the plates were air dried after blocking with BSA.
Biofilms were allowed to form on silicone elastomer discs to early (6 h) or mature (48 h) phases and quantified by tetrazolium (XTT) and dry weight assays.
