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A dye, as a model for small molecular drug, was inserted into carbon nano test tubes (35 nm in diameter, 4.5 μm in length) synthesized using nanochannels of an anodic aluminum oxide film as a template, and the open mouths of the test tubes were sealed by polystyrene polymer.
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The timed test starts when a toothpick coated with the suspected drug is inserted into a vial inside a thick, transparent plastic bag.
Drugs are inserted by their names as reported in DrugBank (49).
Another PE 50 catheter for drug administration was inserted into the right jugular vein.
At the time of the experiment, a cannula for drug administration was inserted and extended another 3.8 mm ventrally beyond the tip of the guide cannula, aiming at the VTA.
Three days before the experiments, rats were anesthetized with halothane (2 3 %) and immobilized at the head, then a 9-cm polyethylene tube (PE-10) for drug delivery was inserted into the cisterna magna, with the tip placed so as to coincide with the lumbar spine (L1 2) according to the methods of Yaksh and Rudy (1976).
A central venous catheter (Certofix Trio V715; B Braun, Melsungen, Germany) for drug and fluid infusion was inserted through the left jugular vein.
To enable drug application, a guide cannula was inserted into the lateral cerebral ventricle.
A central venous catheter was inserted for drug and fluid infusion.
Each week, a random subset of cows in each treatment was fitted with a temperature data logger (HOBO Water Temp Pro v1, Onset Computer Co., Bourne, MA, USA) attached to a blank control internal drug release insert (Pfizer Animal Health) that was inserted into the vagina.
12 We compared operators' performance by analysing endoscopic video recordings and extracting data from their clinical records on need for help, drugs given, distance the endoscope was inserted (for flexible sigmoidoscopy), duration of examination, and immediate complications.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com