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This knowledge will help in drug selection and treatment optimization.
Positive drug selection was done using 2.66 nM WR99210 for pCC1 transfection.
In our system, a transient expression system is used, and continuous drug selection is not necessary.
Nakatake, Y. et al. Kinetics of drug selection systems in mouse embryonic stem cells.
After drug selection, GFP-positive colonies were picked up, and one clone was used for the following experiments.
Stable expression of exogenous proteins was generated by lentiviral or retroviral infection followed by appropriate drug selection.
Culture was maintained for 3 4 weeks in the absence of drug selection with daily medium changes.
The cells were passaged several times and the drug selection was continued for approximately 5 7 days.
The researchers found significant differences between the drug selection habits of Americans and customers from Canada and Western Europe.
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Efficacy is the most important criteria for P-drug selection.
Personal or P-drug selection is an important exercise.
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