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We expect that polymeric ODN delivery after LbL assembly will result in improved therapy due to augmented nuclease stability, high drug loading, efficient delivery, and low cytotoxicity.
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However, there are still several notable problems in the determination of the drug loading content (DLC) and the drug loading efficiency (DLE) of the drug delivery vehicles.
Drug loading content and drug loading efficiency was then determined spectrophotometrically at 303 nm.
The theoretical drug loading content (DLCtheoretical), actual drug loading content (DLCactual), and drug loading efficiency (DLE) were calculated from Equations 2, 3, and 4, respectively.
To achieve efficient drug delivery to a desired site and to reduce the dose, side effects, and total cost of the formulation, drug loading efficiency is desired to be high enough.
Loading efficiency (LE%) was calculated by the following equation: (1) Drug loading efficiency % = Drug total − Drug free Drug total × 100.
Drug loading content, drug loading efficiency, and drug release behavior under reductive condition were studied using ultraviolet spectroscopy.
High drug load efficiency and great drug stability were found.
Nanoparticles have a specific capacity for drug loading, have efficient photoluminescence ability, and are therefore important materials in the targeted delivery of imaging agents and anticancer drugs.
Our results suggest that the aptamer exhibiting a specific intermediate affinity is the best suited to achieve high drug loading while maintaining efficient drug release and therapeutic activity.
Liposome nanocarriers (LPNs) are potentially the future of inner ear therapy due to their high drug loading capacity and efficient uptake in the inner ear after a minimally invasive intratympanic administration.
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