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At appropriate intervals 24 H, 48 H upon treatment with the drug, cells were harvested.
Drug: Cells were left either untreated (0) or were treated with three different concentrations of chemotherapy (1, 2, 3).
After 78 h incubation in the presence of the drug, cells were harvested and total protein was extracted and normalized.
Immediately after the addition of drug, cells were pulsed with [S]-methionine to monitor the production of newly synthesized proteins.
To further confirm the inhibitory effect of drug, cells were cultured with efavirenz for 72 h, followed by growth in drug-free media for 48 h (Fig. 4b).
To measure the retention of drug, cells were incubated with [C]rucaparib for 30 min then washed and transferred to fresh medium, and the intracellular [C]rucaparib concentration was determined as above.
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They should not be using these scarce resources to try to flip Katie and take down one drug cell among roughly 50,000 nationwide.11.11
Drug cell survival curves were performed at pHe values of 6.4, 6.8 and 7.4.
After three days of incubation with the drug, cell viability was measured using the Alamar Blue assay (Invitrogen).
(We will use the concept "intervention" to refer to both what intervenes (the drug, cell line, tissue, device, etc).
DEX1, DEX2, DEX3: Non-drug treated cells (Drug 0) or drug-treated cells (Drug 1, 2, 3) were treated with three different concentrations of DEX.
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