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Four week-old WT and transgenic plants were further supplied with 150 mM NaCl for 20 days for salinity stress, and water withheld for 14 days were used for drought test.
Plants were then subjected to progressive drought test by withholding water for specified times or rosette leaves of similar developmental stages (third to fifth true rosette leaves) were detached and placed abaxial side up on open Petri dishes and weighed at different time intervals at room temperature to determine transpiration rate.
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OBC carried out the mutant screening, physiological drought tests and wrote the manuscript.
For the soil-grown plant drought tolerance test, one-week-old seedlings were transplanted to the soil for two weeks under standard growth conditions, and then plants were subjected to progressive drought by withholding water for specified times.
Drought tolerance test was examined in soil, 7-day-old seedlings were transplanted from MS plates to the soil for another 2 weeks under normal growth conditions.
For each gene construct, 30 T1 families with expression of transgene were selected for drought resistance testing at the reproductive stage in field, and 10 of them were tested in PVC pipes with a defined stress protocol at the same stage.
A drought is testing the state's ancient water infrastructure and threatening farms.
When seedlings regrew well, the drought-stress test was performed.
These data suggest that both local and systemic signaling are likely involved in redirecting lateral roots deeper during the simulated drought treatments tested here.
Drought stress testing at the panicle development stage (~two weeks before flowering) was performed in a paddy field in a greenhouse.
The accumulation of HEL transcripts in response to drought was tested on 80 Recombinant Inbred Lines (RILs) from the cross Ofanto x Cappelli exposed to drought at tillering stage.
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CEO of Professional Science Editing for Scientists @ prosciediting.com