Chromosomes were counted in a drop of immersion oil at 100× using a Leica DM 2500 microscope equipped with a DFC 290 HD camera, using the Leica ver. 3 application suite software.
Following filtration, filters were placed on slides, a drop of immersion oil added to the surface, and a cover slip placed on top.
To obtain the best visibility of the microvasculature, a drop of immersion oil was placed on the nailfold bed.
The nail-fold capillaries were made transparent by adding a drop of immersion oil, and images were generated by a lens providing a magnification of 200×.
A small drop of immersion oil (Immersol 518 F, Zeiss) was placed on the slide in the center of the lighted area.
The membranes were placed on a microscope slide with a small drop of immersion oil, and a cover slip was placed on top.
A drop of immersion oil was placed on top of the filter membrane, and a cover slip was pressed firmly on the oil, with the oil forming a seal at the edge.
A glass cover slip was placed on the mixture, a drop of immersion oil added and the bacteria were inspected in a light microscope with 100× magnification, phase-contrast and a green filter.
Wear gloves and lab coat (the colorant is mutagenic), add a drop of phosphate buffer over the simple (from the 10 mL syringe reserved earlier), place the cover slip avoiding bubble formation, surround it with a clean lint and press gently, take it under the microscope under the 100x lens, add a drop of immersion oil, and observe.
Place a drop of immersion oil on the slide, avoiding motion during application to prevent bubbles.
