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The present work describes an efficient one step green synthesis of 4-arylidene-2-phenyl-5(4H -oxazolones (azlactones) via the multi-component synthesis by the mechanochemical grinding of glycine, benzoyl chloride, an aromatic aldehyde and fused sodium acetate in the presence of drops of acetic anhydride.
A few drops of acetic acid were added finally to get a complete dissociation of zinc acetate.
The aminoalkyl-polyfluorenes have good solubility in organic solvents and also are able to dissolve in methanol by adding a few drops of acetic acid.
An ethanolic solution of 3-amino-2-methylquinazolin-4 3H -one 3-amino-2-methylquinazolin-4 3H -one 3-amino-2-methylquinazolin-4 3H -one 3-amino-2-methylquinazolin-4 3H -one 3-amino-2-methylquinazolin-4 3H -one 3-amino-2-methylquinazolin-4 3H -one 0.005
Benzoyl chloride, glycine, various aromatic aldehydes and fused sodium acetate were mixed under mechanochemical conditions in a porcelain mortar at room temperature in the presence of few drops of acetic anhydride to afford azlactones 2a i.
A mixture of glycine (1.0 mmol), aromatic aldehyde (1.0 mmol), benzoyl chloride (1.0 mmol) and fused sodium acetate (1.0 mmol) was mixed in a porcelain mortar and pestle in the presence of a few drops of acetic anhydride for a few minutes (Table 1).
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The vehicle injection was saline with one drop of Tween 80 and one drop of acetic acid, and was injected 20 minutes before testing (i.e. the approximate harmonic mean of the intervals before behavioural testing).
To another portion of the filtrate was added a drop of acetic anhydride and the color noted.
1 mg of plant extract in methanol was mixed with 1 ml of aluminium trichloride in Ethanol (20 mg/ml) and a drop of acetic acid was added.
Blank samples were prepared from 1 ml of plant extract and a drop of acetic acid, and then diluted to 25 ml with ethanol.
Chromosome spreads were prepared by squashing a stained meristem in a drop of acetic acid (60%%) under the coverslip, and analysed using AxioImager M2 microscope (Carl Zeiss, Vienna, Austria).
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