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At Syrrx, a structural genomics company, a robot places a tiny drop of protein into 480 wells, each containing a different chemical combination.
A 2- μl drop of protein extract is applied onto nitrocellulose membrane and let to dry during 6 8 h.
Tissue fragments were overlaid with a 1 μl drop of protein solution (10 to 400 ng/μl) and assayed for reporter activity, 1 up to 10 days later.
The efficacy of two protocols was compared on explants, either by direct addition of an overlying drop of protein conjugate or by implantation of conjugate-coated beads.
AFM samples were made by applying a drop of protein solution on a 10 × 10 mm hydrophilic silicon wafer (Siltronic Corp).
In the tissue glue group, one drop of thrombin solution was applied over the bare sclera in the recipient bed and one drop of protein concentrate solution was applied over the stromal side of the graft.
Similar(45)
Cover tissue sections with 3-4 drofs of protein block buffer (DAKO) and incubate slides for 15 minutes in humid chamber at room temperature.
Remarkably, BNIP3 silencing led to a drop of the protein levels of the integrin-associated protein CD47 and its downstream signaling effectors Rac1 and Cdc42.
Grids were floated on drops of 1% BSA for 10 min to block for unspecific labeling, transferred to 5 µl drops of primary antibody and incubated for 30 min. The grids were then washed in 4 drops of PBS for a total of 15 min, transferred to 5 µl drops of Protein-A gold for 20 min, washed in 4 drops of PBS for 15 min and 6 drops of double distilled water.
A 10 µl drop of the protein sample was deposited on freshly cleaved mica, allowed to stand for 5 min in air, and then washed with two 100 µl aliquots of distilled deionized water, before drying for 5 min in a stream of nitrogen.
The best crystals were obtained by equilibrating a 1.5 µL drop of a protein (6 mg/mL) in buffer (10 mM HEPES, 50 mM NaCl, 1 mM DTT, pH 7.0), mixed with 1.5 µL of reservoir solution containing 13% (w/v) PEG 6000, 0.2 M ammonium chloride, 20% glycerol, and 0.1 M sodium acetate (pH 5.0) and suspended over 1 mL of reservoir solution.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com