Sentence examples for drop of medium from inspiring English sources

The morphology of the embryo was recorded and then it was cultured under oil in a 30 µl drop of medium (Medicult Universal or Cook's Cleavage Medium) at 37°C in an atmosphere of 5 6% CO2 in air until transfer.

The internal parchment membrane of the air chamber was dissected, and a small silicone ring was placed on the blood vessel area, and 5 × 106 of tumor cells suspended in 20 μl drop of medium were placed into the silicone ring.

A 25  μl drop of medium containing 5 × 10 cells was pipetted onto the inner surface of a Petri dish lid.

Liver fragments were plated 12 fragments per plate covered by a small drop of medium, allowed to adhere and warm medium was added after 2 h.

The lungs were cut into small pieces and 3 6 pieces/well were placed in a six-well plate (Nunc, Denmark) together with a drop of medium and incubated at 5% CO2 and 37°C for 20 min to allow the cells to adhere.

After zona-intact oocytes were treated with collagenase (Wako Pure Chemical Industries, Ltd). at the final concentration of 1 mg/ml in a 30 µl drop of medium (Yamatoya et al., 2011) for 20 min at 37°C, denuded oocytes were removed from the drop with a microcapillary attached to a mouth piece and the remaining solution (<30 µl) was collected as extracellular components of oocytes.

Oocytes were examined for the presence of pronuclei and polar bodies 18 20 h post insemination (p.i .. Normally fertilized eggs with two pronuclei and two polar bodies were either cultured under oil in 30 µl drops of medium (Medicult Universal Medium until May 2005, Cook's Cleavage Medium thereafter) at 37°C in an atmosphere of 5 6% CO2 in air, or frozen immediately.

Infections were done in 30 µl drops of medium containing 5% FBS.

Therefore, few drops of medium were poured into each well before inserting a ChM piece in a well.

Sperm were preincubated in M16 for 60 min and added to drops of medium as in the IVF experiments.

To set-up the cultures, 30  μl drops of medium were prepared on the lid of a Petri-dish (NUNC cell culture Petri-dish).