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Chromosomes were counted in a drop of immersion oil at 100× using a Leica DM 2500 microscope equipped with a DFC 290 HD camera, using the Leica ver. 3 application suite software.
Following filtration, filters were placed on slides, a drop of immersion oil added to the surface, and a cover slip placed on top.
To obtain the best visibility of the microvasculature, a drop of immersion oil was placed on the nailfold bed.
The membranes were placed on a microscope slide with a small drop of immersion oil, and a cover slip was placed on top.
The nail-fold capillaries were made transparent by adding a drop of immersion oil, and images were generated by a lens providing a magnification of 200×.
A small drop of immersion oil (Immersol 518 F, Zeiss) was placed on the slide in the center of the lighted area.
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A few drops of immersion oil were added directly on the completely dry slide, and the cells were examined by phase contrast microscopy (Leica DM5000B).
Samples were allowed to air-dry, mounted with one drop of nonfluorescent immersion oil (Merck) and covered with coverslips.
Cover the tissue slice with one drop of Olympus immersion oil type-F (Olympus, Japan, IMMOIL-F30CC).
To reconstruct and quantify liver tissue, a confocal laser scanning microscope (Olympus, Germany, FV1000) was used and z-stacks were generated as follows: Cover the tissue slice with one drop of Olympus immersion oil type-F (Olympus, Japan, IMMOIL-F30CC).
A particle brought into the interface of a drop (without gravity) achieved its expected depth of immersion into the drop, demonstrating that all aspects of the code work together to produce the correct equilibrium state when a particle is in the interface.
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