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Suspect Salmonella colonies were mixed with a drop of antiserum on a slide.
One drop of this suspension was mixed with 1 drop of antiserum and 1 drop of methylene blue and, after incubation at room temperature for 10 min, examined at 1,000x magnification.
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To assess whether little SAAS is required for intercellular communication within the SCN during glutamatergic phase delay, the SCN was pre-incubated (10 min) with a 1 µL drop of undiluted little SAAS antiserum and then treated with glutamate (1 µL drop, 10 mM), both applied directly to the SCN in the brain slice.
My discipline, molecular cell biology, is expensive: half a millilitre of antiserum can set you back £400, and you can't whip up your own version out of everyday ingredients.
Fig. 6 The curve of antiserum titer detected by indirect ELISA.
Titers of antiserum withdrawn from GNP-injected mice against corresponding antigens are shown in filled columns.
Delivery of antiserum directed against each immunogen conferred varying degrees of protection.
The titer of antiserum was monitored by ELISA.
A comprehensive description of antiserum production and specificity tests is given below.
The rabbits were bled six weeks after the third injection for preparation of antiserum.
The specificity of antiserum to HEX was previously described [ 8].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com