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For example as shown in Figure 10A, a collection of 10 test data points (5 random startle points for air-blow, low drop, medium drop, high drop, and shake, as well as their corresponding 5 rest samples) was used to cross-validate the predictive power of models constructed using all other points (training).
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In order to measure the rate of soluble fibronectin depletion by aggregates in hanging drops, medium was collected after 3, 4, or 5 days in culture.
Liver fragments were plated 12 fragments per plate covered by a small drop of medium, allowed to adhere and warm medium was added after 2 h.
The morphology of the embryo was recorded and then it was cultured under oil in a 30 µl drop of medium (Medicult Universal or Cook's Cleavage Medium) at 37°C in an atmosphere of 5 6% CO2 in air until transfer.
Cells in the exponential phase of growth or heat-killed cells (negative control) were washed and resuspended in drop assay medium (MSB containing 0.2% bacto-agar and 10 mM succinate as an energy source) and poured in Petri plates.
Throughout all experiments, cultures and biofilms were grown at 30°C in M9-AADO (Amino Acid Drop Out) medium without lysine, containing 50 µg ml−1 kanamycin and 20 µg ml−1 tetracycline to maintain the engineered and the marker plasmids, respectively [34].
Transformants were selected in a minimal synthetic drop out medium lacking leucine (amino acid used as auxotrophic marker).
A 25 μl drop of medium containing 5 × 10 cells was pipetted onto the inner surface of a Petri dish lid.
Yeast strains transformed with JRW13 were grown at 26°C to logarithmic phase in an appropriate synthetic drop out medium containing 2% glucose.
Instead, lightly drop the medium into each hole.
S. cerevisiae strains harboring plasmids were grown in synthetic drop-out medium (0.67% yeast nitrogen base, 0.2% amino acid drop out mix and 2% maltose), supplemented with amino acids except the nutrient of the plasmid marker.
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