Sentence examples for drop dilution from inspiring English sources

The purified AS-protease was then refolded by drop dilution method to recover its activity.

The solubilised protein was purified on Ni-NTA Affinity Chromatography (Figure 5B) and then refolded by drop dilution.

The purified protein with urea was then refolded in 20 mM Tris buffer by drop dilution method (Howarth et al. 2006).

The crystallization experiment, containing a control drop, dilution series and the stock solution for each nanosphere size, was pipetted twice.

For drop dilution assays, series of tenfold dilutions derived from a starting solution of 1×108 conidia per ml were spotted in aliquots of 3 µl onto plates.

The experiments including the control drop, dilution series and the stock solution of each nanosphere size were pipetted at least twice for rMaL.

Fractions containing the His-tagged gSG6 were pooled, concentrated by Amicon® Ultra (Millipore, 10 kDa cut-off), refolded by dilution drop-by-drop in 20 volumes of refolding buffer (100 mM Tris-HCl pH 8.0, 500 mM L-Arg, 300 mM NaCl, 5 mM L-Glutathione reduced, 0.5 mM L-Glutathione oxidized) and left for an additional 24 to 48 h at 4°C with low stirring.

Control drops, various dilutions (including 1∶1, 1∶4, 1∶19, and 1∶49) and stock solutions of 20 nm, 50 nm, and 100 nm nanospheres were pipetted in duplicate for SDMT.

Of the 53 patients who had a second convalescent-phase specimen collected after 6 weeks, 16 (31.2%) of 53 showed a 4-fold (2 dilution) drop by week 12 postinfection.

Although we have not quantitatively compared the pressure drop due to dilution and that occurs during solute efflux, we surmise, based on a phenomenological analyses following Idiart and Levin (Idiart and Levin 2004, Levin and Idiart 2004), that pressure drop is more drastic during solute efflux.

This drop presumably reflected dilution of the BrdU label due to multiple rounds of cell division (see below), which implies that most, if not all, of the BrdU/ Tis21-GFP double-positive cells observed aTis21-GFP double-positivenjecTis21-GFP double-positive.