Sentence examples for drop culture from inspiring English sources

ES cells were cultured for 7 d in a hanging drop culture system and allowed to form aggregates called EBs, which were subsequently transferred to gelatin-coated dishes.

The hanging drop culture method represents a variant of 3D culture whereby the cells are cultured in media forming droplets, with no cell attachment to the surroundings, which is often used to produce spheroids from embryonic stem cells in vitro (Wang and Yang 2008).

However, Flk1 expression in microwell chip was markedly lower than that in hanging drop culture, suggesting that microwell chip culture promotes differentiation of hepatic and cardiac lineages.

The expression levels of differentiation markers except Flk1 in the chip with a diameter of 1000 μm were similar to those in hanging drop culture.

So, instead of trying to grow the cells the usual way, in a flat, one-cell layer on a petri dish, he turned to an older method called the "hanging drop culture".

The hanging drop culture was sealed with petroleum jelley to prevent evaporation of the culture medium.

Additionally, all these studies use different kinds of media and culture conditions, e.g. solid agar-media, liquid surface culture or drop cultures, which render direct comparisons difficult.

Hanging drop cultures were prepared using 25 µl droplets each having 600 cells to initiate embryoid body (EB) formation [29].

We measured the concentration of sFn after 72 hours of incubation in hanging drop cultures originally containing 30-µg/ml 30-µg/ml

Inspection of drop cultures containing isolated pairs of mating CCT (α or δ) heterokaryons revealed exconjugant cells that separated but failed to give rise to vigorous clones.

Hanging drop cultures were prepared as described above but at concentrations of either 2.5×106 cells/ml or 5.0×106 cells/ml generating aggregates containing 25,000 tor 50,000 cells.