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In earlier studies, the assay of PHA depolymerase activity on PHA film has been investigated by drop assay of the enzyme on thin films of PHA layered on the surface of solid medium ( Schirmer et al. 1993 ).
We next evaluated the effect of meprinβ on aggregation of suspended cells in a hanging drop assay [35].
Cells in the exponential phase of growth or heat-killed cells (negative control) were washed and resuspended in drop assay medium (MSB containing 0.2% bacto-agar and 10 mM succinate as an energy source) and poured in Petri plates.
The strain sensitivity was tested by the drop assay.
Mouse ES cells were differentiated in vitro by hanging drop assay.
In order to confirm this observation, hanging drop assay was performed.
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The drop assays on solid medium showed that all the transfected E. coli grew well on the selective medium with high K+ level, indicating a successful transfection of these vectors.
Blood smear and thick drop assays were stained with 10% Giemsa dye.
Assays such as wound healing, transwell motility, invasion assays and hanging drop assays have been developed which provide readouts of cellular phenotypes related to metastasis [ 5- 7].
As a further assay of the potential differential cohesion/adhesion properties of eye field versus other neural plate cells, we performed hanging drop assays with cells derived from 100% epiboly stage Tg{rx3 GFP} transgenic embryos.
For metal tolerance assays, yeast was grown in liquid SD medium (with 2% [w/v] galactose) at 30°C until O.D. 600 nm = 1, and drop assays were performed on SD plates containing different concentrations of Zn2+ or Mn2+.
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