This is particularly important when the reporters are to be used in different cell types or in cells from different species, since the efficiencies of the promoters driving selection markers vary [13] [15].
In the study described here, we compared the effects of three different promoters in driving selectable markers for rice transformation.
Previous work comparing different promoters driving selectable markers suggested their possible effects on the copy numbers of the transgene [ 38].
However, our current view of RA as a single disease may become untenable, and the disease may be subdivided into a range of disorders based on improved knowledge of its driving immunologic markers [ 1- 3].
High-frequency gene targeting was obtained using zinc finger nuclease (ZFN -mediated double-strand break (DSB) formation at a pre-defined target genomic location using a unique intron directly downstream of a promoter driving a selectable marker gene to facilitate homology between target and donor sequences.
The cDNAs encoding different semaphorins were subcloned into the NSPI-CMV-MCS-myc-His lentiviral expression vector containing SV40 promoter driving Puromycin selection marker.
The corresponding cell count for fruGAL4 driving a nuclear marker is 950 [ 9].
The effect of a weak promoter driving a selectable marker on the elevation of transformation copy number and transgene expression was also confirmed by using the nopaline synthase (Nos) promoter.
In this report, we demonstrate that a fragment (designated as tCUP1) derived from tCUP was active in a tissue specific manner in rice transgenic plants and behaved as a weak promoter, driving a selectable marker gene for rice transformation.
The tCUP1 promoter allowed the recovery of transformed rice plants and conferred tissue specific expression of the GUS reporter, but was much weaker than the CaMV 35S promoter in driving a selectable marker for growth of resistant calli.
