After demonstrating that the RNA-Seq data was highly sensitive, we then wanted to integrate it with the spatial-resolution embryonic kidney microarray atlas and interrogate the transcriptional complexity driving mouse kidney organogenesis.
We have chosen to use the goat uromodulin promoter driving the GFP kidney-specific expression in transgenic mice as a pilot feasibility study for the goat.
But to Kidney, the driving force was something subtler.
As Notch is involved in regeneration and stem cell functions, we thought that this pathway could have a beneficial effect in kidney disease, maybe driving some processes of healing in response to kidney damage.
Taken together our results demonstrate an essential role for Lhx1 in driving specification of the entire kidney field from the intermediate mesoderm.
The Wnt/ β-catenin canonical pathway plays an essential role during development, driving to branching nephrogenesis in fetal kidney.
The goat uromodulin promoter is capable of driving recombinant protein expression in the kidney of transgenic mice.
Shown in Additional file 3: Figure S1 are proteins and signalling events, assembled to depict probable cascades driving both, apoptosis and necrosis in kidney tissue after injury.
Predicting cancer-specific survival from resection of a T1 renal mass from a solitary kidney may not be driving the oncologic outcome if the same patient had a contralateral nephrectomy for a T3 disease.
