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A starting point for peak lists for database searching was generated by compiling list of peaks that covary with the driver peak by ±half the covariance of the driver peak with itself.
The remaining peaks had both significantly high correlation and a strong covariance with the driver peak.
In 1D versions of STOCSY and SHY, correlations and covariance are determined for a specific "driver" peak of interest.
This was done to eliminate resonances that have a high correlation but a low covariance with the driver peak.
Analysis of the H STOCSY using 1.30 ppm as a driver peak indicated a correlating resonance or overlapping resonances at 3.02 ppm, often assigned to creatine.
As an example, a slice of the 2D C H SHY with C driver peak 31.69 ppm, which is the β carbon of proline, shows correlations with many H resonances other than the reference proline resonances (black), making unambiguous proline identification using H alone difficult.
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The separated driver peaks are then used to re-estimate each individual skin conductance response.
Peak lists from all driver peaks were compared, and those sharing more than three peaks (both H and C) were combined and classified as part of the same metabolite.
We systematically examined all C frequencies along the vertical STOCSY axis now considered "driver peaks"—for every C and H resonance along the horizontal axes of the STOCSY and SHY, respectively.
Also, in my opinion, F1 drivers tend to peak between the ages of 29 and 32.
It is the latter which is the largest driver of peak electrical loads.
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