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To examine the emergence of directional selectivity, we developed genetic driver lines for the neuron types with the most synapses onto T4 cells.
Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgenic driver lines in zebrafish.
The use of specific driver lines to target the alveolar myofibroblast progenitors will be instrumental to follow their fate in the context of lung injury or regeneration.
We extended this approach to additional driver lines.
To date, an increasing number of cell-type restricted driver lines have been generated [12].
The transgenic constructs were tissue-specifically expressed using the GAL4 driver lines described in the text.
Tissue specific expression of the transgenes was achieved by crossing these lines with various driver lines.
Most of the driver lines were provided by the Bloomington center.
To understand the reasons of the difference between mitfa and kita driver lines in developing melanoma, we studied the cell types that express the oncogene under the control of the two driver lines.
UAS-Rheb flies and the GMR-Gal4 and elav-Gal4 driver lines were obtained from the Bloomington stock centre.
The GMR driver lines used a pentameric arrangement of an enhancer region of the Rh1 promoter (glass binding site).
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