Sentence examples for driven vector from inspiring English sources

Any endo-hydrogenase driven, vector H+ translocation, an energy-requiring process, would be necessarily slow by comparison with exo-hydrogenase activity, uncoupled from H+ translocation, and thus relatively fast.

In order to investigate the cellular localization of the putative SAMD9 protein, the open reading frame of the SAMD9 gene was cloned, an N-terminal EGFP tag was added and subcloned into a CMV driven vector pLP-EGFP-C1 (Clontech, USA).

To validate our reporter gene assay and rule out any technical differences in luciferase activity in NALM6 vs. CCRF-CEM cells, we assayed a CMV promoter-luciferase driven vector (pCMV-luc) and found no significant differences in both cell lines (data not shown).

Although at lower efficiency then achieved with the EF1α driven vector, SM22 driven expression was significantly higher in TE-671 cells when compared to HEK293 pointing at the specificity of this construct.

The CMV driven vector pEPito-CMV-EGFPLuc served as positive control achieving the highest luminescence signal, followed by the hCMV/AFP-construct, the hCMV/HPGL-construct and the much weaker respective APOE-derivative.

When the pCLPG vector was employed for transfer of the p53 cDNA, a positive feedback regulatory mechanism was established that both drove vector expression and also blocked tumor cell proliferation [ 22].

In some cases, a double promoter system has also been used to drive vector-based siRNA expression, where two promoters are in tandem or in opposite directions (11- 13).

We cloned seven randomly selected genomic regions (including the four tested in EMSA) (Additional file 6: Table S5) in a minimal promoter driven luciferase vector (pGL4.23) and co-transfected it with the V5-tagged Bapx1 vector into NIH3T3 cell lines.

Expression levels of the recombinant enzymes from a T7 driven pET vector system varied moderately.

With the exception of the promoter region, this construct is identical to the Nrf-2 driven luciferase vector.

Importantly, our conclusions are not confounded by differences in transfection efficiency of reporter constructs because they are reproduced to the same degree in HeLa cells stably expressing the NF-κB driven luciferase vector.