Sentence examples for driven from the endogenous from inspiring English sources
The two versions of tagged receptors have different modes of expression (knocked-in driven from the endogenous promotor vs. transgenic driven from the TH promotor) and different antibodies are used for these two models (rabbit polyclonal anti-GFP vs. mouse monoclonal anti-FLAG M1) minimizing the possibility that the present results are an artifact of the technique.
Since the detection of endogenous Fmn1-IV has been difficult with the existing reagents, we generated an EGFP-Fmn1-IV fusion knock-in mouse in which the fusion protein is driven from the endogenous promoter (Fig. 1A).
Similar results were observed herein utilizing models of cyclin D1b expression driven from the endogenous promoter.
Therefore, transcription of the targeted Cre-ERT2 recombinase will be driven from the endogenous Col1a1 locus and enhanced by the addition of a CAG promoter.
They employ a Tet-On inducible mouse model in which the rtTA is driven from the endogenous ROSA26 locus and leads to mosaic expression in the various blood cell lineages, including the haematopoietic stem cell (HSC; Wörtge et al, 2010).
Although this model is useful, it is important to note that the inducible Kras is encoded by a transgene, therefore resulting in an extra copy of Kras not driven from the endogenous promoter.
To minimize interference with DAT function by preservation of both DAT alleles, Cre recombinase expression was driven from the 3'-untranslated region (3'UTR) of the endogenous DAT gene by means of an internal ribosomal entry sequence (IRES).
To minimize interference with gene function by preservation of both alleles, Cre recombinase expression was driven from the 3'-untranslated region (3'UTR) of the endogenous DAT gene by means of an IRES sequence.
Expression of Erg1 was driven from the strong constitutive promoter glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) replacing the endogenous ERG1 promoter.
