A DNA fragment containing the three promoters drove expression to these same regions and similar results were obtained by in situ hybridization.
The difference in GFP distribution may be explained if trafficking of proteins through PDs is receptor-coupled and SUC2 promoter driven expression leads to overloading of receptors.
While pcDNA-luc (CMV) and pGL3-control (SV40) driven expression was shown to be temperature independent, the influenza replicon system turned out to be highly temperature sensitive.
The SAP2 promoter was induced to drive expression of FLP recombinase to excise the NAT marker cassette.
Constructs synthesized using the minimal Cab promoter to drive expression of GUS were used to transform tomato to assess expression.
For example the Hs278 enhancer drives expression to the hindbrain and spinal cord in transgenic mice, whereas transgenic zebrafish have only spinal cord expression.
However, with C2 attached (Fig. 1) or in the absence of C2, the construct IR V2 V1 drives expression to similar levels (data not shown).
Promoters with tissue-specificity are desirable to drive expression of transgenes in crops to avoid accumulation of foreign proteins in edible tissues/organs.
The Gal4/UAS system is based on the yeast transcription factor Gal4, which binds to 17 to 23 bp UAS [ 11] to drive expression of effector genes/transgenes.
In order to assess this, we used the NP2721-GAL4 line to drive expression of a temperature-sensitive, dominant negative Dynamin, Shibirets (Shits) to block vesicle recycling in DPMs.
